Genome Sequencing

Sequencing Services

 

 

MRDNA Genome Sequencing CostFrom Bacterial and Fungal genome sequencing to human Whole Genome Sequencing (WGS), MR DNA has you covered!!

 

MRDNA has a wide array of NGS platforms to accomplish your sequencing goals, including the Illumina NovaSeq 6000, Illumina HiSeq, and the PacBio Sequel.

 

So no matter the project size, large or small, MR DNA is dedicated to offering our customers the flexibility to achieve the desired library size and desired sequencing coverage at the lowest cost possible.

 

 

 

Pricing

 

Only have a few samples to sequence? No problem!!

     - Bacterial Genomes can be sequenced for as low as $500 and discounts are available for projects with > 5 genomes

 

     - Mid-range size projects (50 - 100 small genomes) can be sequenced for as low as $400 depending on the options needed.

 

You have a big study with a large number of samples? Great!!

     - Bulk pricing for small genomes (> 100 genomes) begins at $350 per sample

     - Contact us for more information on bulk discounts

 

Small Eukaryote genomes start at $600 in our genome service, see below for more information and example programs.

 

 

 

 

 

Sequencing Platform Read Length /Assay Length Genome Sequencing Pricing
Illumina NovaSeq 6000 2x150bp 10 Million PE (Paired-End) sequences / sample = $400 (data only)
30 Million PE (Paired-End) sequences / sample = $800 (data only)
70 Million PE (Paired-End) sequences / sample = $1200 (data only)
     
Illumina NovaSeq 2x250bp 10 Million PE (Paired-End) sequences / sample = $650 (data only)
4 Million PE (Paired-End) sequences / sample = $450 (data only)
     
Illumina MiSeq 2x300 bp 1-2 Million PE (Paired-End) sequences / sample = $600 (data only)
     
PacBio Sequel 10-Hour Movie (Run) Time * Low Coverage Bacterial Genomes starting at $800

* Price will vary based on sequencing coverage required

 

 

Shotgun sequencing and de novo assembly of genomes at MR DNA.

 

MR DNA has many programs for sequencing DNA and RNA (RNAseq, metagenomes, metatranscriptomes,  eukaryote genomes, prokaryote transcriptomes,  bacterial genomes, virus, phage, plasmid, fosmid etc.    (see our transcriptome sequencing page for more on RNA sequencing)

 

BULK AND LARGE PROJECT DISCOUNTS AVAILABLE:  below are program examples for small projects and minimum scale sequencing, with only a few samples, but definitely with larger numbers of samples or samples requiring much more data we definitely can help and provide volume and bulk discounting for some of the lowest price fastest turnaround commercial, academic and government rates available anywhere!

 

Here are just a few examples of shotgun sequencing programs but do contact us for customized projects especially if you have more than a few samples or need larger amounts of data .. we are here to work hard and help!

 

 

Genomes or Metagenome shotgun DNA for small projects:

Large project discounts available

- 2x150bp 10 million paired sequences per sample = $400 (data only) Illumina NovaSeq

- 2x150bp 30 million paired sequences per sample = $800 (data only) Illumina NovaSeq

- 2x150bp 70 million paired sequences per sample = $1200 (data only) Illumina NovaSeq

 

-2x300bp 1-2 million sequences = $600  (data only)  Illumina NovaSeq

-2x250bp 10 million sequences = $650(data only) Illumina NovaSeq

-2x250bp  4 million sequences = $450 (data only)  Illumina NovaSeq

 

Long read sequencing up to 8000bp (or more) available on the PacBio Sequel!   contact us for custom pricing on any project.

 

NEW:  gap closure sequencing long read scaffolding for bacteria = $800/sample.. this adds long read data to illumina sequencing data and may result in much fewer contigs during hybrid assembly.

 

BIOINFORMATICS FOR GENOMES

Data assembly with optimization = $150  (for bacterial genomes we also provide free genome annotation giving you genbank files, proteome files, transcriptome files and genome spreadsheets)

 

 

DNA Extraction / RNA Extraction Price:

--DNA Extraction starting at $25 / sample (depending on service requested and sample type submitted)

--RNA Extraction starting between $30 - $70 / sample (depending  on service requested and sample type submitted)

 

 

Our aim is to deliver research teams with their desired amount of data at the lowest cost possible. We understand that project sizes can vary; from probative studies containing a small number genomic samples to large-scale population studies containing > 100 genomes, and we are here to work with you! With high-output sequencers such as the Illumina HiSeq 2500 and the PacBio Sequel at your disposal, you can rest assured that we will do our best to deliver the maximum output at the lowest cost to you. The prices and data capabilities below represent the typical requests we receive from customers. As members of the scientific community, we understand that not every project is the same. So whether you need 6Tb of data or maybe a more affordable price to complete your large study, don't hesitate to give us a call to see how we can meet your specific needs.

 

 

 

 

Benefits of Whole Genome Sequencing

 

  • Ability to provide greater insight into personalized medicine and diagnosis
  • Aid to uncover the genetic causality of rare and/or inherited diseases
  • Provides researchers with a more balanced coverage in comparison to Whole Exome Sequencing

 

Whole genome sequencing allows scientists to elucidate the entirety of the genome of a specific organism. As the cost of DNA sequencing continues to decrease, due in large part to Next Generation Sequencing, the more affordable and cost effective whole genome sequencing becomes for researchers everywhere. Our experience in whole genome sequencing, paired with a comprehensive portfolio of sequencing platforms, allows us to provide you with the proper work flow for any project. From bacterial genomes to the human genome, we are dedicated to providing our customers with the most reliable and affordable sequencing solution possible. Feel free to contact us for more information.

 

Why Should I Choose Whole Genome Sequencing?

 

Genome Sequencing CostTen years ago this would have been a valid question, and to some regard still is, but a more fitting question for the age of technology we find ourselves in today is, "Why NOT choose whole genome sequencing?". Ten years ago, according to the NIH, the cost per genome hovered right around $10M, but due to scientific and technological advancements the $1,000 genome is now a reality. What used to be reserved for large corporations and research projects receiving astronomical funding is now available to even the smallest university labs. A major impact upon the cost of whole genome sequencing is the advancement of sequencing technology itself. High-throughput sequencing, also known as next-generation sequencing (NGS), has made whole genome sequencing largely accessible due to its capability for producing millions of reads. The capability of NGS instruments, such as the Illumina HiSeq, to produce millions of reads is what truely unharnesses to the power of whole genome sequencing; deep sequencing coverage.

 

One key benefit to choosing whole genome sequencing (WGS) over targeted sequencing methods is that WGS allows researchers an all-inclusive look at the genome in question. Where previous sequencing technologies and other methods lack, NGS platforms excel. High-throughput sequencing allows researchers to detect small and rare genetic mutations such as INDELs and SNPs. With the increased sensitivity that NGS platforms bring to whole genome sequencing, the field of personal medicine is exploding. Cutting-edge research is being performed in fields such as Oncology and Immunology with the goal in mind of not only treating a certain cancer or auto-immune disease as a whole, but formulating a treatment plan based on the individual with that certain diagnosis.

 

Now don't limit yourselves to only thinking that WGS is specifically for large genomes . Due to the scalability of certain NGS platforms, the power of whole genome sequencing can be applied to research of  bacteria, fungi, and other microbial organisms. The sequencing depth achieved by NGS platforms allows researchers to truly take advantage of whole genome sequencing when it comes to sequencing small genomes, such as bacterium, because several bacterial genomes can be sequenced in parallel saving both time and money.

 

 

 

In The News

  In a recent interview conducted by Illumina the Illumina NovaSeq is highlighted as a key instrument in the of future agriculture genomics. According to Pew Research, "By 2100, the world’s population is projected to reach approximately 10.9 billion", and a large question for farmers across the globe is, "How are we going to feed everyone?" By utilizing sequencing instruments such as the HiSeq 2500 and the NovaSeq 6000, researchers are able to undertake research projects involving genomes such as wheat, cotton, corn, sorghum, etc. As high throughput sequencers, such as the NovaSeq 6000, continue to grow in their data generation capabilities, the cost of whole genome sequencing continues to decrease, and as a result, WGS of plant species is now commonplace. From identifying novel SNPs to genotyping fungal and insect pathogens, NGS and whole genome sequencing will continue to revolutionize the agrigenomics industry year after year.

 

 

References

Illumina.com. (2019). Agriculture Throughout the World is Benefiting from the Latest NGS Technologies. [online] Available at: https://www.illumina.com/science/customer-stories/icommunity-customer-interviews-case-studies/johnson-agrilife-interview-ag-novaseq.html [Accessed 5 Jul. 2019].

 

Cilluffo, A., Ruiz, N. G., Cilluffo, A., & Ruiz, N. G. (2019, June 17). World population growth is expected to nearly stop by 2100. Retrieved from https://www.pewresearch.org/fact-tank/2019/06/17/worlds-population-is-projected-to-nearly-stop-growing-by-the-end-of-the-century/

 

Why Should I Choose Whole Genome Sequencing?

 

Genome Sequencing CostTen years ago this would have been a valid question, and to some regard still is, but a more fitting question for the age of technology we find ourselves in today is, "Why NOT choose whole genome sequencing?". Ten years ago, according to the NIH, the cost per genome hovered right around $10M, but due to scientific and technological advancements the $1,000 genome is now a reality. What used to be reserved for large corporations and research projects receiving astronomical funding is now available to even the smallest university labs. A major impact upon the cost of whole genome sequencing is the advancement of sequencing technology itself. High-throughput sequencing, also known as next-generation sequencing (NGS), has made whole genome sequencing largely accessible due to its capability for producing millions of reads. The capability of NGS instruments, such as the Illumina HiSeq, to produce millions of reads is what truely unharnesses to the power of whole genome sequencing; deep sequencing coverage.

 

One key benefit to choosing whole genome sequencing (WGS) over targeted sequencing methods is that WGS allows researchers an all-inclusive look at the genome in question. Where previous sequencing technologies and other methods lack, NGS platforms excel. High-throughput sequencing allows researchers to detect small and rare genetic mutations such as INDELs and SNPs. With the increased sensitivity that NGS platforms bring to whole genome sequencing, the field of personal medicine is exploding. Cutting-edge research is being performed in fields such as Oncology and Immunology with the goal in mind of not only treating a certain cancer or auto-immune disease as a whole, but formulating a treatment plan based on the individual with that certain diagnosis.

 

Now don't limit yourselves to only thinking that WGS is specifically for large genomes . Due to the scalability of certain NGS platforms, the power of whole genome sequencing can be applied to research of  bacteria, fungi, and other microbial organisms. The sequencing depth achieved by NGS platforms allows researchers to truly take advantage of whole genome sequencing when it comes to sequencing small genomes, such as bacterium, because several bacterial genomes can be sequenced in parallel saving both time and money.

 

 

 

Sequencing Platform Read Length /Assay Length Genome Sequencing Pricing
Illumina NovaSeq 6000 2x150bp 10 Million PE (Paired-End) sequences / sample = $400 (data only)
30 Million PE (Paired-End) sequences / sample = $800 (data only)
70 Million PE (Paired-End) sequences / sample = $1200 (data only)
     
Illumina NovaSeq 2x250bp 10 Million PE (Paired-End) sequences / sample = $650 (data only)
4 Million PE (Paired-End) sequences / sample = $450 (data only)
     
Illumina MiSeq 2x300 bp 1-2 Million PE (Paired-End) sequences / sample = $600 (data only)
     
PacBio Sequel 10-Hour Movie (Run) Time * Low Coverage Bacterial Genomes starting at $800

Genome Sequencing Cost

Genome Sequencing Cost