MR DNA specializes in any type of amplicon sequencing.

 

From 16s, 18s, ITS, functional genes to any type of custom primer amplicon assay.

We have an extensive in-house assay (primer) collection for 16s sequencing, 18s sequencing,  ITS sequencing, functional genes such as nirS, nifH, dsr,  pufM, nosZ, HMC,  and many more.  if you have a custom assay we inexpensively set it up for you.

Custom assay no problem! 

 

Amplicon(bTEFAP®) Sequencing Prices.

 

MR DNA has all the major sequencing platforms and we have a large selection of amplicon sequencing programs ..  Everything can be customized to the needs of the customer, only limited by the capabilities of the sequencing technologies.

 

Any amplicons (bTEFAP® services) such as 16s, 18s, ITS, functional or custom assays.. if you have an amplicon with custom primers we can help sequence it.

 

Illumina MiSeq and NovaSeq amplicons

examples for any of our hundreds of inhouse assays

***2x300bp PE illumina 20,000 sequence diversity assays

$80/assay 1-20 assays (note: for projects < 10 assays per library, a $100 library fee is added),

$75 for 20-50 assays,

$70 for 50-100 assays,

$65 for 100-150,

$60 for > 150 assays.

additional discounts for very large projects also

 > 300 samples per assay = $55/assay

 >500 samples additional discounts may apply

 

 

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  PACBIO SEQUEL:

  Sequel long read amplicon sequencing for 16s, 18s, ITS and custom amplicons

  MR DNA now accepts any size project (projects with < 10 samples do have a $150 indexing fee and a $20/sample additional PCR replication fee.)

   

  Academic and Government Pricing:

  Low average coverage ( < 500 sequences average per sample)  for $50/sample

  $90 per sample  for 5,000 sequences per assay,

  $125 per sample for 10,000 sequences per assay

  $200 per sample for 20,000 sequences per assay

  $300 per sample for 40,000 sequences per assay

   

  Custom scales can have as much data per sample as desired in multiples of 40K with price linearly increased.

   

   

  custom assays from 700bp - 3000bp or larger of course have a barcoding that is typically $15/barcode

   

  DNA Extraction / RNA Extraction:

  --DNA Extraction starting at $30 / sample (depending on service requested and sample type submitted)

  --RNA Extraction starting between $40 - $70 / sample (depending  on service requested and sample type submitted)

   

  1)    2x300bp Illumina:  (Price per sample can be lower, depending on how many samples, more samples = lower price per sample) .. you can of course request greater depth. (we can take smaller projects but it may be small delay as we gather enough samples to coordinate with small projects).   We can currently have a variety of 16s assays, fungi, archaea and a few functional genes.. Our system allows us to inexpensively setup new assays and custom assays also

   

  NOTE: CUSTOM assay prices are generally only an additional $10-$20/barcode (one time setup fee).  If you need a custom assay we are happy to get them ordered and tested.

   

  2) LONG READ AMPLICONS up to 8000bp,   with 5,000 avg sequence per sample.  One popular application of this is the near full length 16s sequencing using primers 27F-1492R. Projects less than 10 samples may include an additional library fee. Please contact us for more details.

   

   

  Barcoded amplicon sequencing has become a popular sequencing solution for scientists interested in a specific genomic region.  Amplicon sequencing combined with next-generation sequencing allows for thousands of amplicons across many samples to be prepared simultaneously and indexed within hours and often within a single-run. Due to its popularization, barcoded amplicon sequencing can now be completed through a variety of different processes utilizing several different technologies. MR DNA has been at the forefront of this technology and through calculated fine tuning, we are proud to offer an inexpensive sequencing method known as bTEFAP®. Originally designed to employ 16s eubacterial primers to be sequenced via pyrosequencing technology, we have expanded its low cost sequencing capabilities to include Archaea, Fungi, and functional gene primers compatible to be sequenced across all of our sequencing platforms. The increased flexibility and versatility of bTEFAP® gives us the opportunity to provide our customers with the lowest sequencing cost possible.    Amplicon sequencing has become an essential technique for characterizing microbiomes across diverse ecosystems, using specific genetic markers like 16S rRNA, ITS, and COI to explore bacterial, fungal, and other microbial communities. The 16S rRNA gene is a widely-used marker for bacterial and archaeal profiling, allowing researchers to map microbial diversity in environments ranging from the human gut to soil and ocean microbiomes. By targeting hypervariable regions within the 16S gene, amplicon sequencing can identify microbial species, track shifts in community composition, and assess microbial roles in nutrient cycling, biogeochemical processes, and disease. This high-throughput approach has unlocked new insights into how microbial communities influence ecosystem health and interact with host organisms. For fungal community profiling, the Internal Transcribed Spacer (ITS) regions, especially ITS1 and ITS2, are often selected due to their variability, making them effective for species-level differentiation. Amplicon sequencing of ITS regions enables detailed study of fungal populations in soil, plant roots, and other environments where fungi play key ecological roles, such as in decomposition and symbiosis with plants. In human health, ITS sequencing is applied to analyze the mycobiome in systems like the gut, lungs, and skin, where fungal diversity and composition can impact immune function and susceptibility to conditions like asthma, inflammatory bowel disease, and fungal infections. The application of ITS sequencing thus provides a comprehensive view of fungal diversity and dynamics in both natural and host-associated environments. The cytochrome c oxidase subunit I (COI) gene, commonly referred to as the "barcode" gene, is frequently used to identify animal-associated microbiomes and detect eukaryotic parasites or symbionts. COI sequencing is highly valuable for studying complex food webs, parasite-host interactions, and the influence of animal-associated microbes on their hosts. In marine and freshwater microbiomes, COI amplicon sequencing is instrumental in tracking biodiversity among microeukaryotes and understanding ecosystem responses to environmental stressors such as pollution and climate change. By combining markers like COI, 16S, and ITS, researchers can obtain a holistic view of microbial ecosystems, examining interkingdom interactions and their impacts on host health, environmental stability, and species conservation.Amplicon sequencing has become an essential technique for characterizing microbiomes across diverse ecosystems, using specific genetic markers like 16S rRNA, ITS, and COI to explore bacterial, fungal, and other microbial communities. The 16S rRNA gene is a widely-used marker for bacterial and archaeal profiling, allowing researchers to map microbial diversity in environments ranging from the human gut to soil and ocean microbiomes. By targeting hypervariable regions within the 16S gene, amplicon sequencing can identify microbial species, track shifts in community composition, and assess microbial roles in nutrient cycling, biogeochemical processes, and disease. This high-throughput approach has unlocked new insights into how microbial communities influence ecosystem health and interact with host organisms. For fungal community profiling, the Internal Transcribed Spacer (ITS) regions, especially ITS1 and ITS2, are often selected due to their variability, making them effective for species-level differentiation. Amplicon sequencing of ITS regions enables detailed study of fungal populations in soil, plant roots, and other environments where fungi play key ecological roles, such as in decomposition and symbiosis with plants. In human health, ITS sequencing is applied to analyze the mycobiome in systems like the gut, lungs, and skin, where fungal diversity and composition can impact immune function and susceptibility to conditions like asthma, inflammatory bowel disease, and fungal infections. The application of ITS sequencing thus provides a comprehensive view of fungal diversity and dynamics in both natural and host-associated environments. The cytochrome c oxidase subunit I (COI) gene, commonly referred to as the "barcode" gene, is frequently used to identify animal-associated microbiomes and detect eukaryotic parasites or symbionts. COI sequencing is highly valuable for studying complex food webs, parasite-host interactions, and the influence of animal-associated microbes on their hosts. In marine and freshwater microbiomes, COI amplicon sequencing is instrumental in tracking biodiversity among microeukaryotes and understanding ecosystem responses to environmental stressors such as pollution and climate change. By combining markers like COI, 16S, and ITS, researchers can obtain a holistic view of microbial ecosystems, examining interkingdom interactions and their impacts on host health, environmental stability, and species conservation.Amplicon sequencing is a powerful tool in genomics, used widely across fields for applications ranging from microbial diversity studies to targeted cancer research. In environmental microbiology, for instance, amplicon sequencing of the 16S rRNA gene allows scientists to study microbial communities in soil, water, and extreme environments, offering insights into biodiversity, ecosystem health, and the roles of specific microbes. Using metagenomics and next-generation sequencing (NGS) technology, researchers can profile entire microbial ecosystems without the need for culturing, enabling the discovery of rare and unculturable species. This technology is also applied to study pathogen prevalence, microbial resistance genes, and functional diversity in natural and built environments. In medical research, amplicon sequencing is crucial for investigating the genetic mutations associated with diseases, particularly cancer. By targeting and sequencing specific genes or gene regions, such as BRCA1 and BRCA2 in breast cancer, researchers can identify somatic mutations, copy number variations, and other genetic alterations that drive tumor growth and affect patient outcomes. This approach, known as targeted sequencing or deep sequencing, is especially valuable for liquid biopsies, where circulating tumor DNA (ctDNA) is analyzed to monitor cancer progression or response to therapy. Amplicon sequencing’s high sensitivity allows for the detection of low-frequency variants, making it an indispensable tool in precision medicine and personalized oncology. Amplicon sequencing is also widely used in agricultural genomics and plant breeding. By sequencing specific genes associated with traits like disease resistance, drought tolerance, or yield, researchers can identify and track genetic markers linked to desirable characteristics. This targeted sequencing approach accelerates breeding programs by enabling marker-assisted selection and genetic mapping, which help improve crop performance and resilience. In food safety, amplicon sequencing is used to detect pathogens, allergens, and adulterants in agricultural products. By amplifying and sequencing specific DNA barcodes, researchers and regulatory agencies can ensure food authenticity, traceability, and safety, supporting sustainable and secure food systems worldwide.

   

   

  At Molecular Research MR DNA, we offer cutting-edge amplicon sequencing services that provide unparalleled insights into microbial diversity and functionality. Our services utilize powerful markers such as 16S rRNA for bacteria and archaea, ITS for fungi, and COI for eukaryotic microbes to enable precise taxonomic profiling across diverse environments. For researchers looking to investigate microbial metabolic pathways or ecological roles, we also specialize in sequencing functional gene markers, providing critical insights into environmental, clinical, and industrial microbiomes.

  With our advanced sequencing platforms, we deliver high-resolution data for analyzing complex microbial communities. Our 16S rRNA gene sequencing is ideal for profiling bacteria and archaea in gut microbiomes, soil, and industrial settings. ITS sequencing provides comprehensive fungal community analysis, supporting research into soil health, plant-fungi interactions, and mycobiome studies. COI sequencing enables the exploration of eukaryotic microbial diversity, offering valuable insights into biodiversity, symbiotic relationships, and ecosystem dynamics. Additionally, functional gene analysis sheds light on microbial contributions to nitrogen cycling, carbon metabolism, antibiotic resistance, and other key processes.

  Amplicon sequencing is a cornerstone of microbiome research, and our expertise spans a wide array of applications. For human microbiome studies, our services enable the analysis of gut, skin, and oral microbial communities, aiding in research on health, disease, and personalized medicine. Environmental microbiology applications include characterizing soil, water, and air microbiomes to study ecosystem resilience and pollutant degradation. In agriculture, our sequencing services support the investigation of plant-microbe interactions and soil microbial health to improve crop productivity and stress resistance. By combining state-of-the-art sequencing with expert bioinformatics, we empower researchers to achieve deeper understanding and breakthroughs in microbiome science.

  At Molecular Research MR DNA, we are dedicated to delivering fast, reliable, and high-quality amplicon sequencing solutions tailored to your research needs. Our commitment to excellence ensures that you receive actionable insights and publication-ready data. Whether you are exploring microbial ecosystems in natural environments, agricultural systems, or the human body, our team is here to provide the tools and expertise you need to succeed.